Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2 - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free
    See all Proteasome 20S LMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Proteasome 20S LMP2 aa 100-200. The exact sequence is proprietary.
    Database link: P28065

  • General notes

    Ab250928 is the carrier-free version of ab187645. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250928 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)

    This data was developed using ab187645, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/10000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab187645, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/2000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 21 kDa why is the actual band size different from the predicted?



    This data was developed using ab187645, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    This data was developed using ab187645, the same antibody clone in a different buffer formulation.Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1). Lane 2: PBS instead of Raji lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.
  • Flow Cytometry - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Flow Cytometry - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    This data was developed using ab187645, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Raji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Immunocytochemistry - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Immunocytochemistry - Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    This data was developed using ab187645, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).
  • Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)
    Anti-Proteasome 20S LMP2 antibody [EPR13784] - BSA and Azide free (ab250928)

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