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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)

Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to Proteasome 26S S2/PSMD2
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Proteasome 26S S2/PSMD2 antibody
    See all Proteasome 26S S2/PSMD2 primary antibodies
  • Description

    Goat polyclonal to Proteasome 26S S2/PSMD2
  • Host species

    Goat
  • Specificity

    ab26078 recognises Proteasome 26S, subunit S2 .
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 26S S2/PSMD2 aa 898-908 (C terminal).
    Sequence:

    C VILRKNPNYDL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     This product was previously labelled as Proteasome 26S S2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Images

  • Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Lanes 1-3 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 1 µg/ml
    Lane 4 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : U251 cell lysate
    Lane 3 : KNRK cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 100 kDa



    Detected by chemiluminescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2µg/mL). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. The nuclei were stained with DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

     Cytoplasmic and some nuclear staining is observed.

  • Flow Cytometry - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Flow Cytometry - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Flow cytometry analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL (blue) followed by Alexa Fluor 488 secondary antibody (1µg/mL) (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. 
     
    IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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