Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10967(B)] to PSME1 - BSA and Azide free
Suitable for: ICC, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free
See all PSME1 primary antibodies
Description

Rabbit monoclonal [EPR10967(B)] to PSME1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC, WBmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293T, MCF7 and Jurkat lysates. ICC: MCF7 and HeLa cells.
General notes
Ab249185 is the carrier-free version of ab155091. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249185 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR10967(B)
Isotype

IgG
Research areas

Western blot - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)

All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

This data was developed using ab155091, the same antibody clone in a different buffer formulation.

Lanes 1-4: Merged signal (red and green). Green - ab155091 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab155091 Anti-PSME1 antibody [EPR10967(B)] was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)

This data was developed using ab155091, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)

All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 29 kDa

This data was developed using ab155091, the same antibody clone in a different buffer formulation.
Immunocytochemistry - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)

This data was developed using ab155091, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)

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