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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)

Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Proteasome Activator Subunit 4/PSME4 antibody
    See all Proteasome Activator Subunit 4/PSME4 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 1-50.
    Sequence:

    MEPAERAGVGEPPEPGGRPEPGPRGFVPQKEIVYNKLLPYAERLDAESDL


    Database link: NP_055429.2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa and Jurkat whole cell lysates.
  • General notes

     This product was previously labelled as Proteasome Activator Subunit 4

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab157158 was affinity purified using an epitope specific to Proteasome Activator Subunit 4/PSME4 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Images

  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
    Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
    All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158) at 0.4 µg/ml

    Lane 1 : 293T whole
    cell lysate at 50 µg
    Lane 2 : 293T whole
    cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole
    cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 211 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
    Immunoprecipitation - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)

    Detection of Proteasome Activator Subunit 4/PSME4 by Western Blot of Immunprecipitate.
    ab157158 at 1µg/ml labeling Proteasome Activator Subunit 4/PSME4 in 293T whole cell lysate immunoprecipitated using ab157158 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab157158)

    Immunohistochemistry if human non-small cell lung cancer staining Proteasome Activator Subunit 4/PSME4 with ab157158 at 1/1000. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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