Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab235038)
Key features and details
- Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome Activator Subunit 4/PSME4 antibody
See all Proteasome Activator Subunit 4/PSME4 primary antibodies -
Description
Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 105-198.
Database link: Q14997 -
Positive control
- IHC-P: Human heart tissue and prostate cancer tissues. WB: K562 cell lysate
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General notes
This product was previously labelled as Proteasome Activator Subunit 4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab235038)Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab235038) at 4.5 µg/ml + K562 cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 211 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab235038)Paraffin-embedded human prostate cancer tissue stained for Proteasome Activator Subunit 4/PSME4 using ab235038 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab235038)Paraffin-embedded human heart tissue stained for Proteasome Activator Subunit 4/PSME4 using ab235038 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
