Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome 20S LMP7 antibody (ab82528)

Key features and details

  • Rabbit polyclonal to Proteasome 20S LMP7
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Proteasome 20S LMP7 antibody
    See all Proteasome 20S LMP7 primary antibodies

  • Description

    Rabbit polyclonal to Proteasome 20S LMP7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S LMP7 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P28062
    (Peptide available as ab91637)

  • Positive control

    • WB: A549, Jurkat, Raji and U937 cell lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab82528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Cow
Pig
Non human primates
Application Abreviews Notes
WB
Notes
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 30 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.

    • Involvement in disease

      Defects in PSMB8 are the cause of JMP syndrome (JMPS) [MIM:613732]; also called joint contractures muscular atrophy microcytic anemia and panniculitis-induced lipodystrophy. JBTS1 is an autoinflammatory disorder characterized by childhood onset of joint stiffness and severe contractures of the hands and feet, erythematous skin lesions with subsequent development of severe lipodystrophy, and laboratory evidence of immune dysregulation. Accompanying features include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.

    • Sequence similarities

      Belongs to the peptidase T1B family.

    • Developmental stage

      Highly expressed in immature dendritic cells (at protein level).

    • Post-translational
      modifications

      Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ALDD antibody
        • D6S216 antibody
        • D6S216E antibody
        • Large multifunctional peptidase 7 antibody
        • Large multifunctional protease 7 antibody
        • LMP 7 antibody
        • LMP7 antibody
        • Low molecular mass protein 7 antibody
        • Low molecular weight protein 7 antibody
        • Macropain subunit C13 antibody
        • MGC1491 antibody
        • Multicatalytic endopeptidase complex subunit C13 antibody
        • NKJO antibody
        • OTTHUMP00000062981 antibody
        • Protease component C13 antibody
        • Proteasome (prosome macropain) subunit beta type 8 antibody
        • Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody
        • Proteasome beta 8 subunit antibody
        • Proteasome catalytic subunit 3i antibody
        • Proteasome component C13 antibody
        • Proteasome related gene 7 antibody
        • Proteasome subunit beta 5i antibody
        • Proteasome subunit beta 8 antibody
        • Proteasome subunit beta type 8 antibody
        • Proteasome subunit beta type antibody
        • Proteasome subunit beta type-8 antibody
        • Proteasome subunit beta-5i antibody
        • Proteasome subunit Y2 antibody
        • PSB8_HUMAN antibody
        • PSMB 8 antibody
        • PSMB5i antibody
        • PSMB8 antibody
        • Really interesting new gene 10 protein antibody
        • RING 10 antibody
        • RING10 antibody
        • Y2 antibody
        see all

      Images

      • Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1/500 dilution

        Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
        Lane 2 : PSMB8 knockout A549 (Human lung carcinoma cell line) whole cell lysate
        Lane 3 : Raji (Human Burkitts lymphoma cell line) whole cell lysate
        Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 30 kDa
        Observed band size: 23 kDa
        why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab82528 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab82528 Anti-Proteasome 20S LMP7 antibody was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267149 (knockout cell lysate ab257130) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab82528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1/500 dilution

        Lane 1 : Wild-type A549 cell lysate
        Lane 2 : PSMB8 knockout A549 cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : HEK-293 cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 30 kDa
        Observed band size: 23 kDa why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab82528 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab82528 Anti-Proteasome 20S LMP7 antibody was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267148 (knockout cell lysate ab257129) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab82528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1 µg/ml

        Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
        Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
        Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 30 kDa
        Observed band size: 22 kDa why is the actual band size different from the predicted?
        Additional bands at: 145 kDa. We are unsure as to the identity of these extra bands.



        We hypothesize that the 22 kDa band corresponds to the mature form of Proteasome 20S LMP7.

      References (0)

      Publishing research using ab82528? Please let us know so that we can cite the reference in this datasheet.

      ab82528 has not yet been referenced specifically in any publications.

      Images

      • Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1/500 dilution

        Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
        Lane 2 : PSMB8 knockout A549 (Human lung carcinoma cell line) whole cell lysate
        Lane 3 : Raji (Human Burkitts lymphoma cell line) whole cell lysate
        Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 30 kDa
        Observed band size: 23 kDa
        why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab82528 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab82528 Anti-Proteasome 20S LMP7 antibody was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267149 (knockout cell lysate ab257130) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab82528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1/500 dilution

        Lane 1 : Wild-type A549 cell lysate
        Lane 2 : PSMB8 knockout A549 cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : HEK-293 cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 30 kDa
        Observed band size: 23 kDa why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab82528 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab82528 Anti-Proteasome 20S LMP7 antibody was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267148 (knockout cell lysate ab257129) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab82528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        Western blot - Anti-Proteasome 20S LMP7 antibody (ab82528)
        All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1 µg/ml

        Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
        Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
        Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 30 kDa
        Observed band size: 22 kDa why is the actual band size different from the predicted?
        Additional bands at: 145 kDa. We are unsure as to the identity of these extra bands.



        We hypothesize that the 22 kDa band corresponds to the mature form of Proteasome 20S LMP7.

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