Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TACC3 antibody [EPR7756] (ab134154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7756] to TACC3
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TACC3 antibody [EPR7756]
    See all TACC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7756] to TACC3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TACC3 aa 50-150. The exact sequence is proprietary.
    Database link: Q9Y6A5

  • Positive control

    • WB: Human lymph node tissue lysate. Mouse and rat testis tissue lysate. HEK293T, HCT116, HT-29 and HeLa whole cell lysate (ab150035). IHC-P: Human testis and tonsil tissues. ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7756

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : TACC3 knockout HEK293T cell lysate
    Lane 3 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 90 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab134154 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab134154 Anti-TACC3 antibody [EPR7756]  was shown to specifically react with TACC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266316 (knockout cell lysate ab257723) was used. Wild-type and TACC3 knockout samples were subjected to SDS-PAGE. ab134154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (ab134154)
    Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (ab134154)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with purified ab134154 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/200) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with purified ab134154 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Anti-TACC3 antibody [EPR7756] (ab134154) at 1/2000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified) + Human lymph node tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified)

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)
    All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution (unpurified)

    Lane 1 : Human lymph node tissue lysate
    Lane 2 : HT-29 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (ab134154)
    Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (ab134154)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with unpurified ab134154 at 1/50 dilution.

  • Anti-TACC3 antibody [EPR7756] (ab134154)
    Anti-TACC3 antibody [EPR7756] (ab134154)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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