Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-TACC3 antibody [EPR7756] (ab134154)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7756] to TACC3
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-TACC3 antibody [EPR7756]
See all TACC3 primary antibodies
Description

Rabbit monoclonal [EPR7756] to TACC3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human TACC3 aa 50-150. The exact sequence is proprietary.
Database link: Q9Y6A5
Positive control
- WB: Human lymph node tissue lysate. Mouse and rat testis tissue lysate. HEK293T, HCT116, HT-29 and HeLa whole cell lysate (ab150035). IHC-P: Human testis and tonsil tissues. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7756
Isotype

IgG
Research areas

Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)

All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TACC3 knockout HEK293T cell lysate
Lane 3 : HCT116 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 90 kDa
Observed band size: 125 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab134154 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.

ab134154 Anti-TACC3 antibody [EPR7756] was shown to specifically react with TACC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266316 (knockout cell lysate ab257723) was used. Wild-type and TACC3 knockout samples were subjected to SDS-PAGE. ab134154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (ab134154)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with purified ab134154 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control: primary antibody (1/200) and secondary antibody, ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with purified ab134154 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)

Anti-TACC3 antibody [EPR7756] (ab134154) at 1/2000 dilution (purified) + HeLa cell lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 90 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)

Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified) + Human lymph node tissue lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 90 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)

All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified)

Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 90 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TACC3 antibody [EPR7756] (ab134154)

All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution (unpurified)

Lane 1 : Human lymph node tissue lysate
Lane 2 : HT-29 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TACC3 antibody [EPR7756] (ab134154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (ab134154)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with unpurified ab134154 at 1/50 dilution.
Anti-TACC3 antibody [EPR7756] (ab134154)

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