Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-GSDMA antibody [EPR19858-17] (ab214818)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19858-17] to GSDMA
Suitable for: IHC-Fr, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-GSDMA antibody [EPR19858-17]
See all GSDMA primary antibodies
Description

Rabbit monoclonal [EPR19858-17] to GSDMA
Host species

Rabbit
Specificity

ab214818 also reacts with mouse GSDMA2 and GSDMA3.

Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Hela cells stably transfected with human or mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. Human stomach lysate. Human and rat skin lysate. IHC-Fr: Mouse stomach and breast skin tissue. IP: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19858-17
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (ab214818)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMA with ab214818 at 1/300 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in cells localized in the differentiated region of stomach epithelia of mouse stomach tissue section (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Antigen retrieval is not needed.
Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818)

All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

Lane 1 : HeLa cells, whole cell lysate
Lane 2 : HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate lysate
Lane 3 : HeLa cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5%NFDM/TBS.

The observed molecular mass is consistent with the literature (PMID:17350798).

The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (ab214818)

GSDMA was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab214818 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214818 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 µg (Input).
Lane 2: ab209847 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214818 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: Less than 1 second.

The observed molecular mass is consistent with the literature (PMID:17350798).

The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (ab214818)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse breast skin tissue labeling GSDMA with ab214818 at 1/50 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in skin keratinocytes and hair follicle cells on mouse breast skin, negative staining in basal and stromal cells (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Antigen retrieval is not needed.
Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818)

All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

Lane 1 : Human stomach lysate
Lane 2 : Human skin lysate
Lane 3 : Rat skin lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 49 kDa

Blocking/Dilution buffer: 5%NFDM/TBST.

Exposure times:

Lanes 1/3: 102 seconds

Lane 2: 3 minutes

This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.
Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818)

All lanes : Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : HeLa cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 49 kDa

Exposure time: 70 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-GSDMA antibody [EPR19858-17] (ab214818)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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