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Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19858-104] to GSDMA - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free
    See all GSDMA primary antibodies

  • Description

    Rabbit monoclonal [EPR19858-104] to GSDMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. IHC-P: Human skin tissue; mouse stomach tissue; rat stomach tissue.

  • General notes

    Ab231077 is the carrier-free version of ab230768. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231077 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19858-104

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of rat stomach (PMID: 23979942) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of mouse stomach (PMID: 23979942) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of human skin (PMID: 23979942) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

  • Immunoprecipitation - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)
    Immunoprecipitation - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)

    GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab230768 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230768 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).
    Lane 2: ab230768 IP in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230768 in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: less than 1 second.

    The observed molecular mass is consistent with the literature (PMID:17350798).

    The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

  • Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)
    Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (ab231077)

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