Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Friday March 19, 2021

Anti-GSDMA antibody [EPR19858-116] (ab232254)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19858-116] to GSDMA
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-GSDMA antibody [EPR19858-116]
See all GSDMA primary antibodies
Description

Rabbit monoclonal [EPR19858-116] to GSDMA
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate; Human skin and stomach lysates. IP: HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19858-116
Isotype

IgG
Research areas

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-116] (ab232254)

GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab232254 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab232254 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 µg (Input).

Lane 2: ab232254 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab232254 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

The bands lower than 50 kDa are the degraded exogenous GSDMA protein.

The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Western blot - Anti-GSDMA antibody [EPR19858-116] (ab232254)

All lanes : Anti-GSDMA antibody [EPR19858-116] (ab232254) at 1/1000 dilution

Lane 1 : Human skin lysate
Lane 2 : Human stomach lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 49 kDa

Exposure time : Lane 1: 3 minutes; Lane 2: 102 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

We recommend optimizing experimental conditions when performing WB.
Western blot - Anti-GSDMA antibody [EPR19858-116] (ab232254)

All lanes : Anti-GSDMA antibody [EPR19858-116] (ab232254) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : HeLa cells stable transfected with mouse GSDMA1 expression vector, containing a DDDDK-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 6 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The bands lower than 50 kDa are the degraded exogenous GSDMA protein.

The antibody only recognizes human GADMA.

The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Anti-GSDMA antibody [EPR19858-116] (ab232254)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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