Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)
Key features and details
- Rabbit monoclonal [EP1252Y] to MMP8 - Low endotoxin, Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free
See all MMP8 primary antibodies -
Description
Rabbit monoclonal [EP1252Y] to MMP8 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung lysate. IHC-P: Human urinary bladder carcinoma and placenta tissues. IP: Rat lung lysate.
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General notes
ab224265 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1252Y -
Isotype
IgG -
Research areas
Images
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ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Purified ab81286 at 1/50 dilution (2µg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input): Rat lung lysate 10µg
Lane 2 (+): ab81286 + Rat lung lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286). -
This IHC data was generated using the same anti-MMP8 antibody clone [EP1252Y] in a different buffer formulation (cat# ab81286).
ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.