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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Enzymes MMP

Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

Price and availability

526 012 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP1252Y] to MMP8 - Low endotoxin, Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free
    See all MMP8 primary antibodies
  • Description

    Rabbit monoclonal [EP1252Y] to MMP8 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat lung lysate. IHC-P: Human urinary bladder carcinoma and placenta tissues. IP: Rat lung lysate.
  • General notes

    ab224265 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1252Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Atherosclerotic proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Tumor biomarkers ELISA kits

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)
    Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    Purified ab81286 at 1/50 dilution (2µg) immunoprecipitating MMP8 in Rat lung lysate.
    Lane 1 (input): Rat lung lysate 10µg
    Lane 2 (+): ab81286 + Rat lung lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 70 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free (ab224265)

    This IHC data was generated using the same anti-MMP8 antibody clone [EP1252Y] in a different buffer formulation (cat# ab81286).

    ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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