Anti-MMP8 antibody (ab53017)
Key features and details
- Rabbit polyclonal to MMP8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MMP8 antibody
See all MMP8 primary antibodies -
Description
Rabbit polyclonal to MMP8 -
Host species
Rabbit -
Specificity
MMP8 antibody detects endogenous levels of total MMP8 protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide derived from human MMP8
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MMP8 antibody (ab53017)All lanes : Anti-MMP8 antibody (ab53017) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Predicted band size: 53 kDa
Observed band size: 53 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody (ab53017)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP8 antibody at 1/50 dilution. Left hand image; untreated tissue, Right hand image; tissue treated with the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-MMP8 antibody (ab53017)ICC/IF image of ab63017 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53017, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
