Anti-MMP8 antibody [EP1252Y] (ab81286)
![Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-11.jpg "Anti-MMP8 antibody [EP1252Y] (ab81286)")
Key features and details
- Rabbit monoclonal [EP1252Y] to MMP8
- Suitable for: WB, IHC-P, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP8 antibody [EP1252Y]
See all MMP8 primary antibodies -
Description
Rabbit monoclonal [EP1252Y] to MMP8 -
Host species
Rabbit -
Specificity
WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP RatWB Rat
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Immunogen
A synthetic peptide corresponding to residues in human MMP8.
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Positive control
- WB: Rat lung lysate. IHC-P: Human urinary bladder carcinoma and placenta tissues. IP: Rat lung lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1252Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution + Rat lung lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-13.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/ab81286-4-anti-mmp8-antibody-ep1252y-immunoprecipitation-lung-rat.jpg)
Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)Purified ab81286 at 1/50 dilution (2µg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input): Rat lung lysate 10µg
Lane 2 (+): ab81286 + Rat lung lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-15.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
