Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA-DR antibody [EPR3692] (ab92511)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3692] to HLA-DR
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-DR antibody [EPR3692]
    See all HLA-DR primary antibodies

  • Description

    Rabbit monoclonal [EPR3692] to HLA-DR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HLA-DR aa 150-250. The exact sequence is proprietary.
    Database link: P01903

  • Positive control

    • WB: Raji, Human spleen and Hu T-78 cell lysates IHC-P: Human tonsil and Human kidney tissues

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 1.67 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3692

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)

    ab92511 staining HLA-DR in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] (ab92511)

    ab92511 staining HLA-DR in HuT-78 (human Sezary syndrome) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab92511 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Western blot - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Western blot - Anti-HLA-DR antibody [EPR3692] (ab92511)
    All lanes : Anti-HLA-DR antibody [EPR3692] (ab92511) at 1/10000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma) whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma) whole cell lysate
    Lane 3 : Human tonsil

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 29 kDa



    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Flow Cytometry - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Overlay histogram showing Raji cells stained with ab92511 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92511, 1/50) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Western blot - Anti-HLA-DR antibody [EPR3692] (ab92511)
    All lanes : Anti-HLA-DR antibody [EPR3692] (ab92511) at 1/5000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Human spleen lysate
    Lane 3 : Hu T-78 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)

    ab92511 showing positive staining in Normal liver vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)

    ab92511 showing positive staining in Normal skin vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (ab92511)

    ab92511 showing positive staining in Endometrial carcinoma vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-HLA-DR antibody [EPR3692] (ab92511)
    OI-RD Scanning - Anti-HLA-DR antibody [EPR3692] (ab92511)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-HLA-DR antibody [EPR3692] (ab92511)
    Anti-HLA-DR antibody [EPR3692] (ab92511)

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