Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11226] to HLA-DPB1 - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free
    See all HLA-DPB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11226] to HLA-DPB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal thymus and Human tonsil lysates; Human tonsil tissue; Jurkat cells; Immunoprecipitation pellet from fetal thymus lysate.

  • General notes

    ab215973 is the carrier-free version of ab157210.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab157210 at a working dilution of 1/2500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

  • Immunoprecipitation - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunoprecipitation - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    ab157210 (purified) at 1/70 immunoprecipitating MHC Class II in 10 μg Daudi cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    Immunofluorescence staining of Raji cells with purified ab157210 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab157210 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemical staining of paraffin embedded human tonsil with purified ab157210 at a working dilution of 1/2500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    Unpurified ab157210 showing negative staining in Human heart.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    Unpurified ab157210 showing negative staining in Human normal brain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    Unpurified ab157210 showing positiveve staining in human normal colon.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MHC Class II with unpurified ab157210 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

    Immunofluorescent analysis of Jurkat cells labeling MHC Class II with unpurified ab157210 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157210).

  • Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)
    Anti-HLA-DPB1 antibody [EPR11226] - BSA and Azide free (ab215973)

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