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Anti-HLA-DPB1 antibody [SP229] (ab227676)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP229] to HLA-DPB1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-DPB1 antibody [SP229]
    See all HLA-DPB1 primary antibodies

  • Description

    Rabbit monoclonal [SP229] to HLA-DPB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos, Raji and HeLa cell lysates; human spleen tissue lysate IHC-P: Human tonsil tissue; Flow Cyt: Ramos cells; ICC: Ramos cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by Protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP229

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling HLA-DPB1 with ab227676 at 1/100 dilution (1.10 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Western blot - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    All lanes : Anti-HLA-DPB1 antibody [SP229] (ab227676) at 0.11 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte)
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte)
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell)
    Lane 4 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Blocking/diluting Buffer and concentration: 5% NFDM /TBST
  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [SP229] (ab227676)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DPB1 with purified ab227676 at 1/10 (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Flow Cytometry - Anti-HLA-DPB1 antibody [SP229] (ab227676)

    Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DPB1 with purified ab227676 at 1/20 dilution (5.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (ab227676)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DPB1 using ab227676 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Flow Cytometry - Anti-HLA-DPB1 antibody [SP229] (ab227676)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DPB1 with ab227676 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

  • Anti-HLA-DPB1 antibody [SP229] (ab227676)
    Anti-HLA-DPB1 antibody [SP229] (ab227676)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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