Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [JS76] to HLA DRB1 + HLA-DPB1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA DRB1 + HLA-DPB1 antibody [JS76]

  • Description

    Mouse monoclonal [JS76] to HLA DRB1 + HLA-DPB1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Ramos whole cell lysate. IHC-P: Human tonsil tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    JS76

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA DRB1 + HLA-DPB1 with ab241560 at 0.798µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab241560 for 30 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

  • Western blot - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)
    Western blot - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)
    All lanes : Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560) at 0.399 µg/ml

    Lane 1 : Ramos (Human Burkitt’s lymphoma B lymphocyte) lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 114 seconds.

    Negative control: Hela (PMID: 20959457).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20959457).

  • Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)
    Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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