Anti-GILT antibody (ab232915)
Key features and details
- Rabbit polyclonal to GILT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GILT antibody
See all GILT primary antibodies -
Description
Rabbit polyclonal to GILT -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human GILT aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P13284 -
Positive control
- WB: Raji cell lysate; Recombinant human GILT protein. IHC-P: Human lung, prostate cancer, glioma, stomach, prostate and colorectal cancer tissues. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232915 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GILT antibody (ab232915)Anti-GILT antibody (ab232915) at 3 µg/ml + Recombinant human GILT protein
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
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Western blot - Anti-GILT antibody (ab232915)Anti-GILT antibody (ab232915) at 3 µg/ml + Raji (human Burkitt's lymphoma cell line) cell lysate
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab232915)Paraffin-embedded human lung tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab232915)Paraffin-embedded human prostate cancer stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab232915)Paraffin-embedded human glioma tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab232915)Paraffin-embedded human stomach tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab232915)Paraffin-embedded human prostate tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab232915)Paraffin-embedded human colorectal cancer tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-GILT antibody (ab232915)U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GILT (green) using ab232915 at 20 µg/ml in ICC/IF, followed by FITC staining.
