Anti-GILT antibody (ab224124)
Key features and details
- Rabbit polyclonal to GILT
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GILT antibody
See all GILT primary antibodies -
Description
Rabbit polyclonal to GILT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GILT aa 50 to the C-terminus.
Database link: P13284 -
Positive control
- WB: Human cell line RPMI-8226. IHC-P: Human lymph node tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GILT antibody (ab224124)Anti-GILT antibody (ab224124) at 0.4 µg/ml + Human cell line RPMI-8226
Predicted band size: 29 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-GILT antibody (ab224124)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for GILT (green) using ab224124 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (ab224124)Paraffin-embedded human lymph node tissue stained for GILT using ab224124 at 1/2500 dilution in immunohistochemical analysis.
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Western blot - Anti-GILT antibody (ab224124)All lanes : Anti-GILT antibody (ab224124) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 29 kDa
