Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DP with ab227675 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (11 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DP with ab227675 (green) compared with a rabbit isotype control (blue).

Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.