Anti-HLA Class II DRB1 antibody (ab235953)
Key features and details
- Rabbit polyclonal to HLA Class II DRB1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HLA Class II DRB1 antibody
See all HLA Class II DRB1 primary antibodies -
Description
Rabbit polyclonal to HLA Class II DRB1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human HLA Class II DRB1 aa 1 to the C-terminus.
Database link: Q95IE3 -
Positive control
- WB: A-375 and Raji whole cell lysates. ICC/IF: A-375 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HLA Class II DRB1 antibody (ab235953)All lanes : Anti-HLA Class II DRB1 antibody (ab235953) at 1/1000 dilution
Lane 1 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 30 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody (ab235953)A-375 (Human malignant melanoma cell line) cells stained for HLA Class II DRB1 (Green) using ab235953 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
