Antibodies, Proteins, Kits and Reagents for Life Science

Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Key features and details

Mouse monoclonal [HLA-DRB/1067] to HLA Class II DRB1
Suitable for: IHC-P, WB, Flow Cyt, ICC
Reacts with: Human
Isotype: IgG2b

Product name

Anti-HLA Class II DRB1 antibody [HLA-DRB/1067]
See all HLA Class II DRB1 primary antibodies
Description

Mouse monoclonal [HLA-DRB/1067] to HLA Class II DRB1
Host species

Mouse
Specificity

Reacts with the beta chain.

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Tissue, cells or virus corresponding to Human HLA Class II DRB1. Activated human peripheral blood mononuclear cells.
Database link: P01911
Positive control
- WB: Ramos cell lysate and Human spleen tissue lysate; IHC-P: Human tonsil and histiochytoma tissues; ICC and Flow Cyt: Raji cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

ab215835 is purified from Bioreactor Concentrate by Protein A/G.
Clonality

Monoclonal
Clone number

HLA-DRB/1067
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunocytochemistry - Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Immunocytochemistry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling HLA Class II DRB1 with ab215835 conjugated with APC (Green). The nuclear counterstain is Reddot (Red)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human histiocytoma tissue labeling HLA Class II DRB1 with ab215835 at 1 μg/ml dilution.
Western blot - Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

All lanes : Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Human spleen tissue lysate

Predicted band size: 29 kDa
Flow Cytometry - Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Flow Cytometry analysis of human Raji (human Burkitt's lymphoma cell line) cells labeling HLA Class II DRB1 with ab215835 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling HLA Class II DRB1 with ab215835 at 1 μg/ml dilution.
Western blot - Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835)

Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835) at 1 µg/ml + Ramos cell lysate

Predicted band size: 29 kDa

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