Anti-Scn1a antibody [EPR22326-112] (ab222091)
![Anti-Scn1a antibody [EPR22326-112] (ab222091)](https://www.abcam.com/ps/products/222/ab222091/Images/ab222091-1-anti-scn1a-antibody-epr22326112-immunohistochemistry-mouse-cerebellum.jpg "Anti-Scn1a antibody [EPR22326-112] (ab222091)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22326-112] to Scn1a
- Suitable for: ICC, IHC-Fr, Flow Cyt (Intra), IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-Scn1a antibody [EPR22326-112]
See all Scn1a primary antibodies -
Description
Rabbit monoclonal [EPR22326-112] to Scn1a -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Fr, Flow Cyt (Intra), IHC-Pmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse cerebellum, Mouse hippocampus tissues. IHC-Fr: Mouse hippocampus (fresh) tissue. ICC: Mouse primary neuron cells. Flow Cyt: Mouse primary neuron cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22326-112 -
Isotype
IgG
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab222091)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling Scn1a with ab222091 at 1/1000 dilution (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebellum. The section was incubated with ab222091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab222091)Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labelling Scn1a with ab222091 at 1/1000 dilution (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse hippocampus. The section was incubated with ab222091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab222091)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling Scn1a with ab222091 at 1/500 dilution (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cardiac muscle. The section was incubated with ab222091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Frozen sections) - Anti-Scn1a antibody (ab222091)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling Scn1a with ab222091 at 1/100 dilution (5.4 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
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Immunocytochemistry - Anti-Scn1a antibody (ab222091)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Scn1a with ab222091 at 1/50 dilution (10.8 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
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Flow Cytometry - Anti-Scn1a antibody (ab222091)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Scn1a with ab222091 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Anti-Scn1a antibody (ab222091)