Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MHC Class II beta antibody [EPR11227] (ab170867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11227] to MHC Class II beta
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MHC Class II beta antibody [EPR11227]
    See all MHC Class II beta primary antibodies

  • Description

    Rabbit monoclonal [EPR11227] to MHC Class II beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MHC Class II beta (Cysteine residue). The exact sequence is proprietary.
    Database link: P04440

  • Positive control

    • Human kidney and uterus tissues; Human tonsil, thymus and Raji lysates; Raji cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11227

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II beta antibody [EPR11227] (ab170867)
    Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II beta antibody [EPR11227] (ab170867)

    Immunofluorescent analysis of Raji cells labeling MHC Class II beta with ab170867 at 1/100 dilution (green). DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] (ab170867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] (ab170867)

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human uterus tissue labeling MHC Class II beta with ab170867 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] (ab170867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] (ab170867)

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded human lymphoid follicle labeling MHC Class II beta with ab170867 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-MHC Class II beta antibody [EPR11227] (ab170867)
    Western blot - Anti-MHC Class II beta antibody [EPR11227] (ab170867)
    All lanes : Anti-MHC Class II beta antibody [EPR11227] (ab170867) at 1/1000 dilution

    Lane 1 : Human tonsil lysates
    Lane 2 : Human thymus lysates
    Lane 3 : Raji lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa

  • Anti-MHC Class II beta antibody [EPR11227] (ab170867)
    Anti-MHC Class II beta antibody [EPR11227] (ab170867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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