Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP228] to HLA-DP - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-DP antibody [SP228] - BSA and Azide free
    See all HLA-DP primary antibodies

  • Description

    Rabbit monoclonal [SP228] to HLA-DP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Ramos cells. ICC/IF: Ramos cells.

  • General notes

    ab245736 is the carrier-free version of ab227675.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP228

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)
    Flow Cytometry - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)

    Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227686).

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (11 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101691).

  • Flow Cytometry - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)
    Flow Cytometry - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DP with ab227675 (green) compared with a rabbit isotype control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227675).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DP with ab227675 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227675).

  • Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)
    Anti-HLA-DP antibody [SP228] - BSA and Azide free (ab245736)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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