Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RFXANK antibody [OTI3E7] (ab236408)

Key features and details

Mouse monoclonal [OTI3E7] to RFXANK
Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Reacts with: Human, African green monkey
Isotype: IgG2b

Product name

Anti-RFXANK antibody [OTI3E7]
See all RFXANK primary antibodies
Description

Mouse monoclonal [OTI3E7] to RFXANK
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human RFXANK aa 1 to the C-terminus. Produced in HEK293T cells.
Database link: NP_604389

Run BLAST with Run BLAST with
Positive control
- WB: pCMV6-ENTRY RFXANK cDNA transfected HEK-293T cell lysate. IHC-P: Human kidney, kidney carcinoma, ovary adenocarcinoma, pancreas, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY RFXANK. Flow Cyt: HeLa and Jurkat cells.
General notes

Clone OTI3E7 (formerly 3E7).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI3E7
Isotype

IgG2b
Research areas

Western blot - Anti-RFXANK antibody [OTI3E7] (ab236408)

All lanes : Anti-RFXANK antibody [OTI3E7] (ab236408) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY RFXANK cDNA transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 24, 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human kidney tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human kidney carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human ovary adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human pancreas tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human bladder carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (ab236408)

Paraffin-embedded human tonsil tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (ab236408)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY RFXANK stained for RFXANK (green) using ab236408 at 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-RFXANK antibody [OTI3E7] (ab236408)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
Flow Cytometry - Anti-RFXANK antibody [OTI3E7] (ab236408)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

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