Anti-Apolipoprotein CIII antibody (ab21032)
Key features and details
- Rabbit polyclonal to Apolipoprotein CIII
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apolipoprotein CIII antibody
See all Apolipoprotein CIII primary antibodies -
Description
Rabbit polyclonal to Apolipoprotein CIII -
Host species
Rabbit -
Specificity
Specifically binds to human Apo CIII. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length protein corresponding to Human Apolipoprotein CIII .
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.0292% EDTA, 0.435% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by Human Apo CIII-Sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Apolipoprotein CIII antibody (ab21032)All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml
Lane 1 :Native Human Apolipoprotein CIII (ab77944) at 0.1 µg
Lane 2 :Native Human Apolipoprotein CIII (ab77944) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CIII antibody (ab21032)ICC/IF image of ab21032 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells.
The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa-Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
