Mek1 (pS217/221) + total Mek1 ELISA Kit (ab126448)
Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Mek1 (pS217/221) + total Mek1 ELISA Kit
See all MEK1 kits -
Detection method
Colorimetric -
Sample type
Cell Lysate -
Assay type
Semi-quantitative -
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
ab126448 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Mek1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Mek1 (Ser217/221) and total Mek1 in human, mouse and rat cell lysates (help normalize the results of phospho-Mek1 from different cell lysate being compared). An anti-pan Mek1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Mek1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Mek1 (Ser217/221) or anti-pan-Mek1 antibody is used to detect phosphorylated Mek1 (Ser217/221) or total Mek1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mek1 (Ser217/221) or total Mek1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysis Buffer 1 x 10ml 500X HRP-conjugated anti-rabbit IgG 1 x 25µl 5X Assay Diluent 1 x 15ml Detection Antibody Mek1 1 vial Detection Antibody Mek1 (Ser217/221) 1 vial Mek1 Microplate (12 strips x 8 wells) coated with anti-pan Mek1 antibody 1 unit Positive Control: lyophilized powder from HeLa cell lysate 1 vial Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. -
Tissue specificity
Widely expressed, with extremely low levels in brain. -
Involvement in disease
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Alternative names
- Dual specificity mitogen activated protein kinase kinase 1
- Dual specificity mitogen-activated protein kinase kinase 1
- ERK activator kinase 1
see all -
Database links
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q02750 Human
- SwissProt: P31938 Mouse
- SwissProt: Q01986 Rat
- Unigene: 145442 Human
see all
Images
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Western blot - Mek1 (pS217/221) + total Mek1 ELISA Kit (ab126448)HeLa cells were treated or untreated with TPA for 15 min. Cell lysates were analyzed by Western Blot.
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Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (ab126448)HeLa cells were treated or untreated with TPA for 15 min. Cell lysates were analyzed using ab126448. -
Sandwich ELISA - Mek1 (pS217/221) + total Mek1 ELISA Kit (ab126448)HeLa cells were treated with TPA at 37°C for 15 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of cell lysates were analyzed using ab126448.
