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Recombinant Human MYL1 protein (His tag) (ab275566)

Recombinant Human MYL1 protein (His tag) (ab275566)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human MYL1 protein (His tag)
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    P05976
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      PKKDVKKP VAAAAAAPAP APAPAPAPAP AKPKEEKIDL SAIKIEFSKE QQDEFKEAFL LFDRTGDSKI TLSQVGDVLR ALGTNPTNAE VRKVLGNPSN EELNAKKIEF EQFLPMMQAI SNNKDQATYE DFVEGLRVFD KE
    • Predicted molecular weight

      17 kDa
    • Molecular weight information

      17kDa by SDS-PAGE reducing conditions.
    • Amino acids

      3 to 142
    • Tags

      His tag N-Terminus
  • Specifications

    Our Abpromise guarantee covers the use of ab275566 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.01% Sarkosyl, 5% Trehalose, 0.05% Proclin 300, 0.02% DTT, 0.03% EDTA

    • Reconstitution
      Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

    General Info

    • Alternative names

      • A1 catalytic
      • Alkali myosin light chain 1
      • MLC1/MLC3
      • MLC1F
      • MLC1F/MLC3F
      • MLC3F
      • Myl1
      • MYL1_HUMAN
      • Myosin light chain 1 skeletal muscle isoform
      • Myosin light chain 1/3
      • Myosin light chain 1/3, skeletal muscle isoform
      • Myosin light chain A1/A2
      • Myosin light chain alkali 1/2
      • skeletal muscle isoform
      see all
    • Function

      Regulatory light chain of myosin. Does not bind calcium.
    • Sequence similarities

      Contains 3 EF-hand domains.
    • Post-translational
      modifications

      Isoform MLC3 is acetylated at position 2.
    • Target information above from: UniProt accession P05976 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Images

  • SDS-PAGE - Recombinant Human MYL1 protein (His tag) (ab275566)
    SDS-PAGE - Recombinant Human MYL1 protein (His tag) (ab275566)

    SDS-PAGE analysis of ab275566.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab275566? Please let us know so that we can cite the reference in this datasheet.

    ab275566 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • A1 catalytic
    • Alkali myosin light chain 1
    • MLC1/MLC3

    Images

    • SDS-PAGE - Recombinant Human MYL1 protein (His tag) (ab275566)
      SDS-PAGE - Recombinant Human MYL1 protein (His tag) (ab275566)

      SDS-PAGE analysis of ab275566.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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