Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9938] to Cytochrome C Oxidase subunit VIc/COX6C
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938]
See all Cytochrome C Oxidase subunit VIc/COX6C primary antibodies
Description

Rabbit monoclonal [EPR9938] to Cytochrome C Oxidase subunit VIc/COX6C
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cytochrome C Oxidase subunit VIc/COX6C aa 50-150 (C terminal). The exact sequence is proprietary.
Positive control
- WB: Human fetal heart lysate; HepG2 and A-673 cell lysates. Human heart lysates, Mouse heart lysates and Rat heart tissue lysates IHC-P: Human colon, breast carcinoma, colonic adenocarcinoma, kidney, liver, skeletal muscle and stomach tissues. Mouse cardiac muscle tissue, and Rat liver tissue.
General notes
This product was previously labelled as Cytochrome C Oxidase subunit VIc

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9938
Isotype

IgG
Research areas

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/1000 dilution (Purified)

Lane 1 : Human heart lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 9 kDa
Observed band size: 12 kDa
why is the actual band size different from the predicted?
Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/5000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 9 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1:500 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cardiac muscle tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1:500 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1:500 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/1000 dilution

Lane 1 : Human fetal heart cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : A-673 (Human muscle Ewing's Sarcoma cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab150422 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab150422 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using ab150422 showing positive staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemical analysis of paraffin embedded human liver tissue using ab150422 showing positive staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemical analysis of paraffin embedded human colonic adenocarcinoma tissue using ab150422 showing positive staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

Immunohistochemical analysis of paraffin embedded human colon tissue using ab150422 showing positive staining.
Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422)

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