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Alexa Fluor® 488 Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab198593)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2b

Overview

  • Product name

    Alexa Fluor® 488 Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3]
    See all Cytochrome C Oxidase subunit VIc/COX6C primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Cow Cytochrome C Oxidase subunit VIc/COX6C.

  • Positive control

    • ICC/IF: HeLa cells Flow Cyt: HeLa cells.

  • General notes

     This product was previously labelled as Cytochrome C Oxidase subunit VIc

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab198593)
    Flow Cytometry - Alexa Fluor® 488 Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab198593)

    Overlay histogram showing HeLa cells stained with ab198593 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198593, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b (monoclonal) Alexa Fluor® 488 (ab171465) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab198593)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab198593)

    ab198593 staining Cytochrome C Oxidase subunit VIc/COX6C in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198593 at a 1/500 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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