Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)
![Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-315391-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-western-blot.jpg "Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19140] to TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516)
- Suitable for: Dot blot, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140]
See all TrkA+TrkB+TrkC primary antibodies -
Description
Rabbit monoclonal [EPR19140] to TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag; HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes; PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19140 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution
Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-261152-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-western-blot.jpg)
Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/10000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y516A mutant) with Myc-tag
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
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![Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-261151-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-western-blot.jpg)
Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
-
![Dot Blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-261149-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-dot-blot.jpg)
Dot Blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)Dot blot analysis of TrkA (phospho Y496), TrkB (phospho Y516), and TrkC (phospho Y516) peptides labeled with ab197071 at 1/1000 dilution.
Lane 1: TrkB (phospho Y516) phospho peptide;
Lane 2: TrkB Non-phospho peptide;
Lane 3: TrkA (phospho Y496) phospho peptide;
Lane 4: TrkA Non-phospho peptide;
Lane 5: TrkC (phospho Y516) phospho peptide;
Lane6: TrkC Non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Note: ab197071 reacts with TrkB (phospho Y516)/ TrkA (phospho Y496)/ TrkC (phospho Y516) according to the dot blot data.
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![Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-5-benefits-of-recombinant-antibodies.png)
Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)