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Neuroscience Neurology process Growth and Development Neurotrophins

Recombinant rat CNTF protein (Animal Free) (ab217482)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: HPLC, Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant rat CNTF protein (Animal Free)
    See all CNTF proteins and peptides
  • Biological activity

    Determined by its ability to stimulate proliferation of human TF-1 cells using a concentration range of 25.0-35.0 ng/mL.

  • Purity

    > 98 % SDS-PAGE.
    > 98 % HPLC.
  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    P20294
  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Rat
    • Sequence

      AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
    • Predicted molecular weight

      23 kDa
    • Amino acids

      2 to 200
  • Associated products

    • Related Products

      • Anti-CNTF antibody (ab9785)

    Specifications

    Our Abpromise guarantee covers the use of ab217482 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      Functional Studies

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • Ciliary neurotrophic factor
      • CNTF
      • CNTF_HUMAN
      • HCNTF
    • Function

      CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
    • Tissue specificity

      Nervous system.
    • Sequence similarities

      Belongs to the CNTF family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P26441 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab217482? Please let us know so that we can cite the reference in this datasheet.

    ab217482 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Recombinant rat CNTF protein (Animal Free) (ab217482)

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    • Recombinant human CNTF protein (ab9972)

      Applications:

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    • Recombinant human CNTF protein (ab9786)

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