Recombinant rat CNTF protein (ab129035)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant rat CNTF protein
See all CNTF proteins and peptides -
Biological activity
Measured in a cell proliferation assay using TF1 Human erythroleukemic cells. The ED50 for this effect is typically 3 - 15 ng/ml. -
Purity
> 98 % SDS-PAGE.
Purity determined by SDS-PAGE and HPLC. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLD SVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPT EGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGL FEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQMA FAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDS VDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTE GDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLF EKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM -
Predicted molecular weight
23 kDa -
Amino acids
2 to 200
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Store under desiccating conditions.
Constituent: 0.06% Tris
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Reconstitutionab129035 is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in water to a concentration of 100 µg/ml. This solution can be diluted with water or other buffered solutions or stored at -20°C for future use. Reconstituted ab129035 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Images
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SDS-PAGE - Recombinant rat CNTF protein (ab129035)SDS-PAGE analysis of ab129035.
Sample was loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. -
Functional Studies - Recombinant rat CNTF protein (ab129035)Proliferation assay with TF1 cells.
