Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1294] to TrkB
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-TrkB antibody [EPR1294]
See all TrkB primary antibodies
Description

Rabbit monoclonal [EPR1294] to TrkB
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human TrkB aa 100-200 (internal sequence). The exact sequence is proprietary.
(Peptide available as ab195463)
Positive control
- Fetal brain, Human hippocampus and Human cerebellum lysates; Human brain tissue
General notes
Mouse, Rat: Internal testing data in Western Blot indicate that this antibody is not suitable for these species.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1294
Isotype

IgG
Research areas

Western blot - Anti-TrkB antibody [EPR1294] (ab134155)

All lanes : Anti-TrkB antibody [EPR1294] (ab134155) at 1/1000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : Human hippocampus lysate
Lane 3 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab134155 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab134155 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab134155 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (ab134155)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134155 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TrkB antibody [EPR1294] (ab134155)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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