Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-REC8 antibody [EPR16189] (ab192241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16189] to REC8
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-REC8 antibody [EPR16189]
    See all REC8 primary antibodies

  • Description

    Rabbit monoclonal [EPR16189] to REC8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human, mouse and rat testis and Jurkat lysates; Human, mouse and rat testis tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/10000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa

  • Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa

  • Anti-REC8 antibody [EPR16189] (ab192241)
    Anti-REC8 antibody [EPR16189] (ab192241)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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