Anti-Tristetraprolin/TTP antibody (ab83579)
Key features and details
- Rabbit polyclonal to Tristetraprolin/TTP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Tristetraprolin/TTP antibody
See all Tristetraprolin/TTP primary antibodies -
Description
Rabbit polyclonal to Tristetraprolin/TTP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
-
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Jurkat; Ramos; A431; SK N SH; PC3.
-
General notes
This product was previously labelled as Tristetraprolin
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).Target
-
Function
mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). -
Sequence similarities
Contains 2 C3H1-type zinc fingers. -
Post-translational
modificationsPhosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation. -
Cellular localization
Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules. - Information by UniProt
-
Database links
- Entrez Gene: 7538 Human
- Omim: 190700 Human
- SwissProt: P26651 Human
- Unigene: 534052 Human
-
Alternative names
- G0/G1 switch regulatory protein 24 antibody
- G0S24 antibody
- GOS24 antibody
see all
Images
-
Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)All lanes : Anti-Tristetraprolin/TTP antibody (ab83579) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutesTristetraprolin contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The expression profile observed is also consistent with what has been described in the literature (PMID:16508015).
-
Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)This image is courtesy of an anonymous AbreviewAnti-Tristetraprolin/TTP antibody (ab83579) at 1/1000 dilution + HeLa whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
References (5)
ab83579 has been referenced in 5 publications.
- Cao H & Sethumadhavan K Regulation of Cell Viability and Anti-inflammatory Tristetraprolin Family Gene Expression in Mouse Macrophages by Cottonseed Extracts. Sci Rep 10:775 (2020). PubMed: 31964945
- Li P et al. Tristetraprolin attenuates brain edema in a rat model of cerebral hemorrhage. Brain Behav 9:e01187 (2019). PubMed: 30729695
- Khalaj K et al. A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients. Sci Rep 7:5883 (2017). PubMed: 28724967
- Krishnamurthy PM et al. Involvement of p38-ßTrCP-Tristetraprolin-TNFa axis in radiation pneumonitis. Oncotarget 8:47767-47779 (2017). PubMed: 28548957
- Ray D et al. Tristetraprolin mediates radiation-induced TNF-a production in lung macrophages. PLoS One 8:e57290 (2013). PubMed: 23468959
Images
-
Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)All lanes : Anti-Tristetraprolin/TTP antibody (ab83579) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutesTristetraprolin contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The expression profile observed is also consistent with what has been described in the literature (PMID:16508015).
-
Western blot - Anti-Tristetraprolin/TTP antibody (ab83579) This image is courtesy of an anonymous AbreviewAnti-Tristetraprolin/TTP antibody (ab83579) at 1/1000 dilution + HeLa whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
