Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)
![Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137498-anti-tristetraprolin-antibody-oti8b5-western-blot.jpg "Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)")
Key features and details
- Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP
- Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Tristetraprolin/TTP antibody [OTI8B5]
See all Tristetraprolin/TTP primary antibodies -
Description
Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Tristetraprolin/TTP.
Database link: NP_003398 -
Positive control
- HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA; Human colon, colon adenocarcinoma, ovary, endometrium and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY Tristetraprolin/TTP.
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General notes
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 8B5 to OTI8B5, both clone numbers name the same antibody clone.
This product was previously labelled as Tristetraprolin
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI8B5 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA
Lysates/proteins at 5 µg per lane.
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202049) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137497-anti-tristetraprolin-antibody-oti8b5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137496-anti-tristetraprolin-antibody-oti8b5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137495-anti-tristetraprolin-antibody-oti8b5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human ovary tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137494-anti-tristetraprolin-antibody-oti8b5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human endometrium tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137493-anti-tristetraprolin-antibody-oti8b5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human prostate tissue by Immunohistochemistry.
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![Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137492-anti-tristetraprolin-antibody-oti8b5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024, at 1/100, staining Tristetraprolin/TTP in COS7 cells transiently transfected with pCMV6-ENTRY Tristetraprolin/TTP by Immunofluorescence.
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![Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)](https://www.abcam.com/ps/products/124/ab124024/Images/ab124024-137491-anti-tristetraprolin-antibody-oti8b5-flow-cytometry.jpg)
Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)ab124024 at 1/100 dilution staining Tristetraprolin/TTP in HEK293T cells transfected with either pCMV6-ENTRY Tristetraprolin/TTP (Red) or empty vector control plasmid (Blue) by flow cytometry.
