Anti-nNOS (neuronal) antibody - N-terminal (ab229785)
Key features and details
- Rabbit polyclonal to nNOS (neuronal) - N-terminal
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-nNOS (neuronal) antibody - N-terminal
See all nNOS (neuronal) primary antibodies -
Description
Rabbit polyclonal to nNOS (neuronal) - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Rat nNOS (neuronal) (N terminal). The exact sequence is proprietary.
Database link: P29476 -
Positive control
- IHC-P: Mouse and rat brain tissue; adult mouse cerebellum tissue. WB: Mouse and rat brain tissue extracts. ICC/IF: DIV9 rat E18 primary cortical neurons cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)All lanes : Anti-nNOS (neuronal) antibody - N-terminal (ab229785) at 1/1000 dilution
Lane 1 : Mouse brain tissue extracts
Lane 2 : Rat brain tissue extracts
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 161 kDa5% SDS-PAGE
-
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)DIV9 rat E18 primary cortical neurons cells stained for nNOS (neuronal) (green) using ab229785 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstains: DAPI (blue) and beta Tubulin 3/Tuj1 (red).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)Paraffin-embedded rat brain tissue stained for nNOS (neuronal) with ab229785 at 1/500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)Frozen-sectioned adult mouse cerebellum tissue stained for nNOS (neuronal) with ab229785 at 1/250 dilution in immunohistochemical analysis. NF-H (red), DAPI (blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody - N-terminal (ab229785)Paraffin-embedded mouse brain tissue stained for nNOS (neuronal) with ab229785 at 1/500 dilution in immunohistochemical analysis.
