Antibodies, Proteins, Kits and Reagents for Life Science

Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1855Y] to nNOS (neuronal) - Low endotoxin, Azide free
Suitable for: Flow Cyt (Intra), IP, ICC, WB
Reacts with: Mouse, Rat

Product name

Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free
See all nNOS (neuronal) primary antibodies
Description

Rabbit monoclonal [EP1855Y] to nNOS (neuronal) - Low endotoxin, Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), IP, ICC, WBmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat
Predicted to work with: Human, Common marmoset
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain tissue lysate. ICC: PC-12 cells Flow Cyt (intra): PC-12 cells. IP: Rat brain tissue lysate.
General notes
ab219373 is the carrier-free version of ab76067.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1855Y
Isotype

IgG
Research areas

Immunocytochemistry - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

Immunocytochemistry analysis of PC-12 cells labelling nNOS (neuronal) (green) with purified ab76067 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

ab76067 (purified) at 1/150 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

ab76067 (unpurified) at 1/4 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

Flow cytometry analysis of PC-12 cells labelling nNos (neuronal) with purified ab76067 at 1/600 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

Flow cytometry analysis of PC-12 cells labelling nNos (neuronal) with unpurified ab76067 at 1/15 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

Overlay histogram showing PC-12 cells stained with unpurified ab76067 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab76067, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC-12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373) This image is courtesy of an Abreview submitted by Patricia Martin-DeLeon.

Unpurified ab76067 staining nNOS (neuronal) in murine sperm cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and blocked using 2% BSA. Samples were then incubated with undiluted ab76067. The secondary used was a FITC conjugated goat anti-rabbit IgG at a 1/400 dilution.Panel A shows the specific staining of nNOS in sperm while Panel B is the control sample treated with Rabbit IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
Anti-nNOS (neuronal) antibody [EP1855Y] - Low endotoxin, Azide free (ab219373)

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