Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1855Y] to nNOS (neuronal) - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, WB, ICC
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free
    See all nNOS (neuronal) primary antibodies

  • Description

    Rabbit monoclonal [EP1855Y] to nNOS (neuronal) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WB, ICCmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human, Common marmoset

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: PC-12 Flow Cyt (intra): PC-12 cells. IP: Rat brain tissue lysate.

  • General notes

    ab271857 is the carrier-free version of ab76067.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Immunocytochemistry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

    Immunocytochemistry analysis of PC-12 cells labelling nNOS (neuronal) (green) with purified ab76067 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.  Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).

  • Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

    Overlay histogram showing PC-12 cells stained with unpurified ab76067 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab76067, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC-12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
  • Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

    ab76067 (purified) at 1/150 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
  • Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

    Flow cytometry analysis of PC-12 cells labelling nNos (neuronal) with purified ab76067 at 1/600 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
  • Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Immunoprecipitation - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

    ab76067 (unpurified) at 1/4 immunoprecipitating nNOS (neuronal) in rat brain tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
  • Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Flow Cytometry - Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

    Flow cytometry analysis of PC-12 cells labelling nNos (neuronal) with unpurified ab76067 at 1/15 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76067).
  • Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)
    Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free (ab271857)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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