Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DDAH1 antibody [EPR13921] (ab181859)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13921] to DDAH1
Suitable for: IHC-P, WB, Flow Cyt, IP
Knockout validated
Reacts with: Human

Product name

Anti-DDAH1 antibody [EPR13921]
See all DDAH1 primary antibodies
Description

Rabbit monoclonal [EPR13921] to DDAH1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431, HuVEC, HepG2 and 293 cell lysates. IP: 293 cells. Flow Cyt: 293 cells. IHC-P: Human hepatocellular carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13921
Isotype

IgG
Research areas

Western blot - Anti-DDAH1 antibody [EPR13921] (ab181859)

All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/10000 dilution

Lane 1 : Wild-type A431 cell lysate
Lane 2 : DDAH1 knockout A431 cell lysate
Lane 3 : HuVEC cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Predicted band size: 31 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab181859 observed at 37 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab181859 was shown to react with DDAH1 in wild-type A431 cells in Western blot. Loss of signal was observed when DDAH1 knockout sample was used. Wild-type A431 and DDAH1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181859 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DDAH1 antibody [EPR13921] (ab181859)

All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/5000 dilution

Lane 1 : 293 cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-DDAH1 antibody [EPR13921] (ab181859)

Western blot analysis of 293 cell lysate immunoprecipitated using ab181859 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
Flow Cytometry - Anti-DDAH1 antibody [EPR13921] (ab181859)

Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling DDAH1 with ab181859 at 1/30 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-Rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH1 antibody [EPR13921] (ab181859)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling DDAH1 with ab181859 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-DDAH1 antibody [EPR13921] (ab181859)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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