Human Syndecan-1 ELISA Kit (CD138) (ab46506)
Key features and details
- Sensitivity: 4.94 ng/ml
- Range: 8 ng/ml - 256 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Syndecan-1 ELISA Kit (CD138)
See all Syndecan-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6 6.2% Inter-assay Sample n Mean SD CV% Overall 6 10.2% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 4.94 ng/ml -
Range
8 ng/ml - 256 ng/ml -
Recovery
101 %
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Assay time
1h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
This kit will recognize both endogenous and recombinant Human Syndecan-1.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-Syndecan-1 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl Syndecan-1 Microplate (12 x 8 well strips) 1 unit 2 units Syndecan-1 Standard (lyophilized) Orange 2 vials 4 vials -
Research areas
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Function
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD_antigen
- CD138
- CD138 antigen
see all -
Database links
- Entrez Gene: 6382 Human
- Omim: 186355 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
Images
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ELISA: Syndecan (CD138) Human ELISA Kit (ab46506)
Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).
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ELISA: Syndecan (CD138) Human ELISA Kit (ab46506)Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).
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ELISA: Syndecan (CD138) Human ELISA Kit (ab46506)Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab46506