Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 120 614 ₸

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Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21188] to Myelin Basic Protein
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-Myelin Basic Protein antibody [EPR21188]
See all Myelin Basic Protein primary antibodies
Description

Rabbit monoclonal [EPR21188] to Myelin Basic Protein
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain, cerebral cortex and midbrain tissue lysates; rat cerebral cortex, cerebellum and brain tissue lysates; human cerebellum and brain cortex tissue lysates. IHC-P: Human cerebrum tissue; mouse brain tissue; rat brain tissue; IHC-Fr: Mouse brain (cerebrum) tissue; rat brain (cerebrum) tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21188
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain (cerebrum) tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining in the axonal fiber tracts on rat cerebrum tissue section (PMID: 23144976) is observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris-EDTA buffer, pH 9.0)
Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (cerebrum) tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining in the axonal fiber tracts on rat cerebrum tissue section (PMID: 23144976) is observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris-EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on rat brain (PMID: 23144976) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on mouse brain (PMID: 23144976) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human cerebrum (PMID: 22496821) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Lanes 1-6 : Anti-Myelin Basic Protein antibody [EPR21188] (ab218011) at 1/5000 dilution
Lanes 7-8 : Anti-Myelin Basic Protein antibody [EPR21188] (ab218011) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebral cortex tissue lysate
Lane 3 : Mouse midbrain tissue lysate
Lane 4 : Rat cerebral cortex tissue lysate
Lane 5 : Rat cerebellum tissue lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : Human cerebellum tissue lysate
Lane 8 : Human brain cortex tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 7-8 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 14-21.5 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1-5 & 8: 15 seconds.

Lane 6: 7 seconds.

Lane 7: 32 seconds.
Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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