Anti-Elongation factor 1-gamma antibody (ab72368)
Key features and details
- Rabbit polyclonal to Elongation factor 1-gamma
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Elongation factor 1-gamma antibody
See all Elongation factor 1-gamma primary antibodies -
Description
Rabbit polyclonal to Elongation factor 1-gamma -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Elongation factor 1-gamma aa 200-250.
Database link: NP_001395.1 -
Positive control
- Whole cell lysate from Hela, 293T and NIH3T3 cells.
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General notes
Previously labelled as EEF1G.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Elongation factor 1-gamma antibody (ab72368)All lanes : Anti-Elongation factor 1-gamma antibody (ab72368) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody (ab72368)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Elongation factor 1-gamma with ab72368 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Elongation factor 1-gamma antibody (ab72368)Immunoprecipitation/ Western Blot of Elongation factor 1-gamma. Lane 1: ab72368 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72368 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.
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Western blot - Anti-Elongation factor 1-gamma antibody (ab72368) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Elongation factor 1-gamma antibody (ab72368) at 1/3000 dilution (in PBS-T for 16 hours at 4°C)
Lane 1 : Monkey kidney whole cell lysate overexpressing Elongation factor 1-gamma
Lane 2 : COS7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 10 seconds
Blocking Step: 5% Milk for 1 hour at 22°C -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody (ab72368)IHC image of ab72368 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody (ab72368)ICC/IF image of ab72368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72368, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
