Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7200] to Elongation factor 1-gamma
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Elongation factor 1-gamma antibody [EPR7200]
    See all Elongation factor 1-gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR7200] to Elongation factor 1-gamma

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Elongation factor 1-gamma aa 50-150. The exact sequence is proprietary.

  • Positive control

    • 293T, HL60, C6, RAW 264.7, PC12, NIH 3T3, HepG2 and HeLa cell lysates; Human pancreatic adenocarcinoma tissue; HeLa cells.

  • General notes

     

     

    Previously labelled as EEF1G. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.31 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7200

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    All lanes : Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate
    Lane 7 : HepG2 cell lysate
    Lane 8 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa

  • Flow Cytometry - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Flow Cytometry - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)

    ab124994 at 1/250 dilution staining Elongation factor 1-gamma in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)

    ab124994 at 1/100 dilution staining Elongation factor 1-gamma in HeLa cells by Immunofluorescence.

  • OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
    Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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