Anti-DDX3 antibody (ab151965)
Key features and details
- Rabbit polyclonal to DDX3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DDX3 antibody
See all DDX3 primary antibodies -
Description
Rabbit polyclonal to DDX3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to a region within amino acids 254 and 521 of Human DDX3 (UniProt: O00571)
-
Positive control
- HeLa whole cell, HeLa cytoplasmic or Human embryonic stem cell lysate, Human embryonic stem cells, HeLa cells, Human colon tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-DDX3 antibody (ab151965) at 1/2000 dilution
Lane 1 : HeLa lysate
Lane 2 : HeLa cytoplasmic lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 73 kDa
7.5% SDS PAGE -
Anti-DDX3 antibody (ab151965) at 1/2000 dilution + Human Embryonic stem cell lysate at 20 µg
Predicted band size: 73 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX3 with ab151965 at a 1/250 dilution.
-
Immunofluorescence analysis of paraformaldehyde-fixed Human embryonic stem cells, labeling DDX3 with ab151965 at a 1/200 dilution.
-
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling DDX3 with ab151965 a 1/500 dilution.