Anti-DDX3 antibody (ab235940)
Key features and details
- Rabbit polyclonal to DDX3
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DDX3 antibody
See all DDX3 primary antibodies -
Description
Rabbit polyclonal to DDX3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB MouseHuman
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Immunogen
Recombinant full length protein corresponding to Human DDX3 aa 1 to the C-terminus.
Database link: O00571 -
Positive control
- WB: NIH/3T3, K562, Jurkat and MCF7 whole cell lysatse; Rat brain and heart lysates; Mouse liver and kidney lysates. IHC-P: Human colon cancer, prostate cancer and adrenal gland tissues. ICC/IF: HepG2 cells. IP: Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDX3 antibody (ab235940)All lanes : Anti-DDX3 antibody (ab235940) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Mouse liver lysate
Lane 7 : Mouse kidney lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX3 antibody (ab235940)Paraffin-embedded human prostate cancer tissue stained for DDX3 using ab235940 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-DDX3 antibody (ab235940)HepG2 (human liver hepatocellular carcinoma cell line) cells labeling DDX3 using ab235940 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunoprecipitation - Anti-DDX3 antibody (ab235940)DDX3 was immunoprecipitated from 0.5 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with ab235940 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in Jurkat whole cell lysate.
Lane 2: ab235940 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX3 antibody (ab235940)Paraffin-embedded human adrenal gland tissue stained for DDX3 using ab235940 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX3 antibody (ab235940)Paraffin-embedded human colon cancer tissue stained for DDX3 using ab235940 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Western blot - Anti-DDX3 antibody (ab235940)All lanes : Anti-DDX3 antibody (ab235940) at 1/500 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 73 kDa
