Anti-eIF4E antibody (ab238519)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4E antibody
See all eIF4E primary antibodies -
Description
Rabbit polyclonal to eIF4E -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human eIF4E aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001959.1
Database link: P06730 -
Positive control
- WB: HL-60, SW620, MCF7 and HEK-293T whole cell lysate; Mouse brain lysate; Rat testis lysate. IHC-P: Human lung cancer and stomach tissue. ICC/IF: U-2 OS cells. IP: HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4E antibody (ab238519)All lanes : Anti-eIF4E antibody (ab238519) at 1/1000 dilution
Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : SW620 whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Rat testis tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 10 secondsBlocking buffer: 3% non-fat dry milk in TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody (ab238519)U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for eIF4E (Green) using ab238519 at 1/100 dilution in ICC/IF analysis. Nuclear counterstain was DAPI (Blue).
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Immunoprecipitation - Anti-eIF4E antibody (ab238519)eIF4E was immunoprecipitated from 200 μg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell extract using 3 μg of ab238519. Western blot was performed from the immunoprecipitate using ab238519 at 1/1000 dilution.
Lane 1: HEK-293T cell extract (input).
Lane 2: Control IgG instead of ab238519 in HEK-293T cell extract.
Lane 3: ab238519 IP in HEK-293T cell extract. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab238519)Paraffin-embedded human stomach tissue stained for eIF4E using ab238519 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab238519)Paraffin-embedded human lung cancer tissue stained for eIF4E using ab238519 at 1/100 dilution in immunohistochemical analysis.
