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Human Relaxin-2 ELISA Kit (ab243688)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.05 pg/ml
  • Range: 2.03 pg/ml - 130 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Relaxin-2 ELISA Kit
    See all Relaxin 2/RLN2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    protein 5 6%
    Inter-assay
    Sample n Mean SD CV%
    protein 3 4%

  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.05 pg/ml

  • Range

    2.03 pg/ml - 130 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 89% - 102%
    Cell culture media 83 79% - 85%
    Hep Plasma 87 81% - 91%
    EDTA Plasma 102 97% - 107%
    Cit plasma 104 95% - 110%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Relaxin-2 ELISA Kit (ab243688) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Relaxin-2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Relaxin-2 with 1.05 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Relaxin-2 Capture Antibody 1 x 600µl
    10X Human Relaxin-2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human Relaxin-2 Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

  • Tissue specificity

    Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary.

  • Sequence similarities

    Belongs to the insulin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • H2
    • REL2_HUMAN
    • Relaxin 2
    • Relaxin A chain
    • Relaxin H2
    • RLN2
    • RLXH2
    see all

  • Database links

    Images

    • Other - Human Relaxin-2 ELISA Kit (ab243688)
      Other - Human Relaxin-2 ELISA Kit (ab243688)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human Relaxin-2 Standard Curve Comparison.
      Human Relaxin-2 Standard Curve Comparison.

      Standard Curve comparison between human Relaxin-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

    • Example of human Relaxin-2 standard curve in Sample Diluent NS.
      Example of human Relaxin-2 standard curve in Sample Diluent NS.

      The Relaxin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Relaxin-2 in pooled pregnant human serum samples.
      Interpolated concentrations of native Relaxin-2 in pooled pregnant human serum samples.

      The concentrations of Relaxin-2 were measured in duplicates, interpolated from the Relaxin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Relaxin-2 concentration was determined to be 1,123 pg/mL in pregnant serum.

    • Sandwich ELISA - Human Relaxin-2 ELISA Kit (ab243688)
      Sandwich ELISA - Human Relaxin-2 ELISA Kit (ab243688)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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