Human Relaxin-2 ELISA Kit (ab243688)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.05 pg/ml
- Range: 2.03 pg/ml - 130 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Relaxin-2 ELISA Kit
See all Relaxin 2/RLN2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% protein 5 6% Inter-assay Sample n Mean SD CV% protein 3 4% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.05 pg/ml -
Range
2.03 pg/ml - 130 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96 89% - 102% Cell culture media 83 79% - 85% Hep Plasma 87 81% - 91% EDTA Plasma 102 97% - 107% Cit plasma 104 95% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Relaxin-2 ELISA Kit (ab243688) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Relaxin-2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Relaxin-2 with 1.05 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Relaxin-2 Capture Antibody 1 x 600µl 10X Human Relaxin-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Relaxin-2 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. -
Tissue specificity
Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- H2
- REL2_HUMAN
- Relaxin 2
see all -
Database links
- Entrez Gene: 6019 Human
- Entrez Gene: 6013 Human
- Omim: 179740 Human
- SwissProt: P04090 Human
- SwissProt: P04808 Human
- Unigene: 127032 Human
- Unigene: 368996 Human
- Unigene: 368996 Human
Images
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Other - Human Relaxin-2 ELISA Kit (ab243688)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Relaxin-2 Standard Curve Comparison.Standard Curve comparison between human Relaxin-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Relaxin-2 standard curve in Sample Diluent NS.The Relaxin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Relaxin-2 in pooled pregnant human serum samples.The concentrations of Relaxin-2 were measured in duplicates, interpolated from the Relaxin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Relaxin-2 concentration was determined to be 1,123 pg/mL in pregnant serum.
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Sandwich ELISA - Human Relaxin-2 ELISA Kit (ab243688)To learn more about the advantages of recombinant antibodies see here.
